Journal: Cell reports

Article Title: Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections.

doi: 10.1016/j.celrep.2023.112766

Figure Lengend Snippet: Figure 3. Titration of rtN1- and rtN2-spe- cific serum IgG in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-63His tag Sino Biological Cat#105327-MM02T-H Rabbit polyclonal anti-N1 Sino Biological Cat#11058-R001 Rabbit polyclonal anti-N2 Sino Biological Cat#40017-RP01 HRP-conjugated goat anti-human IgG Proteintech Group Cat#SA00001-17 HRP-conjugated goat anti-mouse IgG Proteintech Group Cat#SA00001-1 HRP-conjugated goat anti-mouse IgG1 Proteintech Group Cat#SA00012-1 HRP-conjugated goat anti-mouse IgG2a Proteintech Group Cat#SA00012-2 HRP-conjugated goat anti-mouse IgG3 Proteintech Group Cat#SA00012-5 HRP-conjugated goat anti-mouse IgM Proteintech Group Cat#SA00012-6 HRP-conjugated peanut agglutinin Sigma-Aldrich Cat#L7759 Bacterial and virus strains E.coli DH5a competent cells Lab stock N/A E.coli DH10Bac competent cells Lab stock N/A A/Hong Kong/8/1968 (H3N2) ATCC Cat#VR544TM A/New Jersey/8/1976 (H1N1) ATCC Cat#VR897TM A/Puerto Rico/8/1934 (H1N1) Haiyan Chang, Hunan Normal University N/A A/reassortant/NYMC X179A (H1N1) Haiyan Chang, Hunan Normal University N/A A/Guizhou/54/1989 (GZ89, H3N2) Haiyan Chang, Hunan Normal University N/A A/Puerto Rico/8/1934 (H1N1) Haiyan Chang, Hunan Normal University N/A Biological samples Human convalescent sera samples Yao-Qing Chen’s laboratory stock N/A Chemicals, peptides, and recombinant proteins N1P1 Top-peptide N/A N1P2 Top-peptide N/A N1P3 Top-peptide N/A N1P4 Top-peptide N/A N2P1 Top-peptide N/A N2P2 Top-peptide N/A N2P3 Top-peptide N/A N2P4 Top-peptide N/A rtN1 This paper N/A rtN2 This paper N/A KLH Solarbio Cat#K8160 Receptor destroying enzyme Denka Seiken Cat#340122 Fetuin from fetal bovine serum Sigma-Aldrich Cat#F2379 Incomplete Freund’s adjuvant Sigma-Aldrich Cat#F5506 Critical commercial assays ReadiLinkTM KLH Conjugation Kit AAT Bioquest Cat#5502 Mouse IFNg ELISPOT Kit BD Biosciences Cat#551083 Mouse IL-4 ELISPOT Kit Dakewe Cat#DKW22-2040-500 (Continued on next page) Cell Reports 42, 112766, July 25, 2023 11

Techniques: Titration, Enzyme-linked Immunosorbent Assay

Journal: Tissue & cell

Article Title: B7-H3 promotes the migration and invasion of colorectal cancer cells via regulating the actin cytoskeleton and RhoA/ROCK1/LIMK1 signaling pathway.

doi: 10.1016/j.tice.2024.102518

Figure Lengend Snippet: Fig. 4. B7-H3 positively regulated actin cytoskeleton and RhoA/ROCK1/LIMK1 pathway (A) The enrichment of molecular function using the differentially expressed genes (DEGs) between negative control (NC) and shB7H3 HCT116 cells. (B) The actin stress fiber formation in HCT116 and RKO cells infected with lentivirus carrying B7-H3 shRNA. (original magnification 100×). (C) The protein expression of RhoA, ROCK1, and LIMK1 in HCT116 and RKO cells infected with lentivirus carrying B7-H3 shRNA. GAPDH served as a loading control. (D, E) The protein expression of RhoA, ROCK1, and LIMK1 in HCT116 (D) and RKO (E) cells infected with lentivirus carrying B7-H3 overexpression vectors. GAPDH served as a loading control.

Article Snippet: The protein bands were incubated overnight with the following primary antibodies: Rabbit anti-human B7-H3 antibody (Proteintech Group, Inc.), rabbit anti-human RhoA antibody (Proteintech Group, Inc.), rabbit anti-human ROCK1 antibody (Proteintech Group, Inc.), rabbit anti-human LIMK1 antibody (Proteintech Group, Inc.) and mouse anti-human LIMK1 antibody (Proteintech Group, Inc.).

Techniques: Negative Control, Infection, shRNA, Expressing, Control, Over Expression

Journal: Tissue & cell

Article Title: B7-H3 promotes the migration and invasion of colorectal cancer cells via regulating the actin cytoskeleton and RhoA/ROCK1/LIMK1 signaling pathway.

doi: 10.1016/j.tice.2024.102518

Figure Lengend Snippet: Fig. 5. Blockade of the RhoA/ROCK1/LIMK1 pathway reversed the effect of B7-H3 on CRC cell migration and invasion (A) The protein expression of LIMK1 in B7-H3 overexpression HCT116 and RKO cells treated with BDP5290. GAPDH served as a loading control. (B) Transwell migration and invasion assays in B7-H3 overexpression HCT116 and RKO cells treated with BDP5290. (C) The actin stress fiber formation in B7-H3 overexpression HCT116 and RKO cells treated with BDP5290. (original magnification 100 ×). The values were represented as means with SD, and the statistical significance was assessed using Student’s t-test. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: The protein bands were incubated overnight with the following primary antibodies: Rabbit anti-human B7-H3 antibody (Proteintech Group, Inc.), rabbit anti-human RhoA antibody (Proteintech Group, Inc.), rabbit anti-human ROCK1 antibody (Proteintech Group, Inc.), rabbit anti-human LIMK1 antibody (Proteintech Group, Inc.) and mouse anti-human LIMK1 antibody (Proteintech Group, Inc.).

Techniques: Migration, Expressing, Over Expression, Control

Journal: Tissue & cell

Article Title: B7-H3 promotes the migration and invasion of colorectal cancer cells via regulating the actin cytoskeleton and RhoA/ROCK1/LIMK1 signaling pathway.

doi: 10.1016/j.tice.2024.102518

Figure Lengend Snippet: Fig. 6. LIMK1 was overexpressed in CRC tissues and positively with the expression of B7-H3. (A) LIMK1 protein expression in adjacent normal tissues (NAT, n = 87) and CRC tissues (n = 94) based on the TMAs. One representative image is shown (scale bar, 200 µm). (B) LIMK1 protein expression based on their staining index in CRC specimens at different AJCC stages. (C) Kaplan−Meier curves of overall survival of CRC patients with high or low LIMK1 expression (log-rank P = 0.0283). (D) Representative B7-H3 and LIMK1 IHC staining images from patients #C3 and #E3 were shown (scale bar, 200 µm). (E) The correlation between B7-H3 and LIMK1 expression in CRC tissues. (F) Kaplan−Meier curves of overall survival of CRC patients according to four subtypes based on B7-H3 and LIMK1 expression. The values were represented as means with SD, and the statistical significance was assessed using Student’s t-test. *P < 0.05 and ***P < 0.001.

Article Snippet: The protein bands were incubated overnight with the following primary antibodies: Rabbit anti-human B7-H3 antibody (Proteintech Group, Inc.), rabbit anti-human RhoA antibody (Proteintech Group, Inc.), rabbit anti-human ROCK1 antibody (Proteintech Group, Inc.), rabbit anti-human LIMK1 antibody (Proteintech Group, Inc.) and mouse anti-human LIMK1 antibody (Proteintech Group, Inc.).

Techniques: Expressing, Staining, Immunohistochemistry